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Literature summary extracted from
- Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli (2001), RNA, 7, 1603-1615.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.20 | D79N | inactive mutant enzyme | Escherichia coli |
| 5.4.99.20 | D79T | inactive mutant enzyme | Escherichia coli |
| 5.4.99.21 | D107N | inactive mutant enzyme | Escherichia coli |
| 5.4.99.21 | D107T | inactive mutant enzyme | Escherichia coli |
| 5.4.99.22 | D110N | inactive mutant enzyme | Escherichia coli |
| 5.4.99.22 | D110T | inactive mutant enzyme | Escherichia coli |
| 5.4.99.26 | D54T | inactive mutant enzyme | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.22 | 23S rRNA uridine2605 | Escherichia coli | - |
23S rRNA pseudouridine2605 | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.20 | Escherichia coli | P75966 | - |
- |
| 5.4.99.21 | Escherichia coli | P32684 | - |
- |
| 5.4.99.22 | Escherichia coli | P37765 | - |
- |
| 5.4.99.26 | Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.20 | 23S rRNA uridine2457 | the enzyme has an essential Asp at postion 79 | Escherichia coli | 23S rRNA pseudouridine2457 | - |
? | |
| 5.4.99.21 | 23S rRNA uridine2604 | it appears that YjbC is not completely specific for uridine2604 but can, to a small extent also react with uridine2605. The enzyme has an essential aspartate residue, at position 107 | Escherichia coli | 23S rRNA pseudouridine2604 | - |
? | |
| 5.4.99.22 | 23S rRNA uridine2605 | - |
Escherichia coli | 23S rRNA pseudouridine2605 | - |
? | |
| 5.4.99.22 | 23S rRNA uridine2605 | the conserved active site residue Asp110 is essential for activity | Escherichia coli | 23S rRNA pseudouridine2605 | - |
? | |
| 5.4.99.26 | tRNA uridine65 | pseudouridine sequencing of the relevant tRNAs isolated from a strain in which yqcB is deleted shows that YqcB specifically forms pseudouridine65 in tRNAAsp and tRNAIle1 | Escherichia coli | tRNA pseudouridine65 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.20 | RluE | - |
Escherichia coli |
| 5.4.99.20 | YmfC | - |
Escherichia coli |
| 5.4.99.21 | RluF | - |
Escherichia coli |
| 5.4.99.21 | YjbC | - |
Escherichia coli |
| 5.4.99.22 | RluB | - |
Escherichia coli |
| 5.4.99.22 | YciL | - |
Escherichia coli |
| 5.4.99.26 | TruC | - |
Escherichia coli |
| 5.4.99.26 | YqcB | - |
Escherichia coli |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.20 | malfunction | pseudouridine2457 is absent in a ymfC deletion strain, it reappears when the deletion strain is transformed by a rescue plasmid carrying the ymfC structural gene | Escherichia coli |
| 5.4.99.21 | malfunction | pseudouridine2604 is absent in the RluF-deletion strain | Escherichia coli |
| 5.4.99.22 | malfunction | no formation of pseudouridine2605 in 23S rRNA of an rluB null mutant. An rluB null mutant or an rluB rluF double null mutant exhibits no obvious growth defect | Escherichia coli |
| 5.4.99.26 | malfunction | pseudouridine sequencing of the relevant tRNAs isolated from a strain in which yqcB is deleted shows that YqcB is the synthase specific for C65 in tRNAAsp and tRNAIle1 | Escherichia coli |
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